Helicobacter pylori infection in Bamako (Mali): Polymorphism, diversity and prophage

Helicobacter pylori (Hp) is a bacteria associated with gastritis and gastric cancer (GC). Its prevalence higher than 80% in Mali affects the incidence of GC, which major department digestive surgery. VacA CagA toxins are responsible Hp strain polymorphism. With prophage, they also region depending diversity. The aim this study was to identify variants strains their prophage identity Bamako (Mali). A total 61 biopsies were isolated patients diseases. extracted DNA amplified by PCR targeting cagA, vacA (s, m) phage integrase (int) genes. cagA int amplicons sequenced according Sanger method. detected 23 (37.7%) samples. predominance housewives, commercial workers, farmers students observed, confirming importance socio-economic conditions factors infection. Carcinogenic vacAs1m1 second dominant polymorph. This would explain Hp's GC country. phylogenetic tree from sequences has revealed specific that causes (HpML). Their integration protein shares more 90% its sequence species Schmidvirus genus. Like HpML probable strains, Malian’s (HpMLpΦ) geographically clustered hspWestAfrica1 (hpAfrica1). These results confirm existence bacteriophage suggest using molecular diagnostics method for effective pathogen identification rapid patient management.